...结合蛋白、模拟表位[45-47]、DNA/RNA-结合蛋白的筛选等方面的问题,其中现代的分子生物学技术如酵母双杂交技术(yeast two-hybrid)[48]、抑制性消减杂交技术(SSH)[49]、噬菌体表面展示(phage display)技术[50]等的应用,都是研究蛋白质的功能与基因的克隆化相耦...
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...ion) :new protein + epitope tag亲和层析法(affinity chromatography) :GST tag fusion 酵母双杂交法(yeast two-hybrid) :Protein Interaction Laboratory of plant m olecular cytogeneticsYeast two-hybrid system 真核转录因子DNA结合结构域(DN...
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yeast two-hybrid system 酵母双杂交系统 ; 酵母双杂交体系 ; 技术 ; 酵母双杂交
yeast two hybrid 酵母双杂交
Yeast-two hybrid technique 酵母双杂交技术
The Yeast two-Hybrid System 酵母双杂交系统
Yeast Two-Hybrid Systems 酵母双杂交系统
Yeast two-hybrid cDNA library 酵母双杂交cDNA文库
reverse yeast two-hybrid system 反向酵母双杂交系统
The yeast two-hybrid system is a novel and sensitive technique to detect protein-protein interactions in vivo.
酵母双杂合系统是在酵母细胞中研究蛋白- 蛋白相互作用的新技术。
CONCLUSION: Yeast two-hybrid GAL4 system 3 can be utilized to fish HCV core region-interacting protein. HCV core protein does not have self-activating function.
结论利用酵母双杂交Gal4系统3研究与HCV核心蛋白相互作用的蛋白,证实了HCV核心蛋白无自激活功能。
Nevertheless, huge protein network is larger than that we predict and single yeast two-hybrid system cannot solve all the problems, which need be complemented by other wags.
但是巨大的蛋白质网络比我们想象要大得多,单一的双杂交系统不能解决所有问题,需要同其它的方法有效地结合。
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